232632

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3269531..3270330 Replicon chromosome
Accession NZ_CP091065
Organism Escherichia coli strain DOC14

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag L1123_RS15960 Protein ID WP_000347273.1
Coordinates 3269866..3270330 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L1123_RS15955 Protein ID WP_001307405.1
Coordinates 3269531..3269866 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1123_RS15940 (3265316) 3265316..3266086 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
L1123_RS15945 (3266102) 3266102..3267436 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L1123_RS15950 (3267811) 3267811..3269382 + 1572 WP_001273753.1 galactarate dehydratase -
L1123_RS15955 (3269531) 3269531..3269866 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L1123_RS15960 (3269866) 3269866..3270330 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L1123_RS15965 (3270385) 3270385..3271194 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L1123_RS15970 (3271443) 3271443..3272723 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
L1123_RS15975 (3272746) 3272746..3273219 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
L1123_RS15980 (3273230) 3273230..3273601 + 372 Protein_3123 PTS sugar transporter subunit IIC -
L1123_RS15985 (3273597) 3273597..3274154 + 558 Protein_3124 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3260383..3270330 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T232632 WP_000347273.1 NZ_CP091065:3269866-3270330 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T232632 NZ_CP127848:746038-746334 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT232632 WP_001307405.1 NZ_CP091065:3269531-3269866 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT232632 NZ_CP127848:746336-746731 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References