27421

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3254908..3255707 Replicon chromosome
Accession NC_017638
Organism Escherichia coli DH1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag ECDH1ME8569_RS16055 Protein ID WP_000347273.1
Coordinates 3255243..3255707 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag ECDH1ME8569_RS16050 Protein ID WP_001307405.1
Coordinates 3254908..3255243 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1ME8569_RS16035 (ECDH1ME8569_3017) 3250693..3251463 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
ECDH1ME8569_RS16040 (ECDH1ME8569_3018) 3251479..3252813 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
ECDH1ME8569_RS16045 (ECDH1ME8569_3019) 3253188..3254759 + 1572 WP_001273747.1 galactarate dehydratase -
ECDH1ME8569_RS16050 (ECDH1ME8569_3020) 3254908..3255243 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
ECDH1ME8569_RS16055 (ECDH1ME8569_3021) 3255243..3255707 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
ECDH1ME8569_RS16060 (ECDH1ME8569_3022) 3255762..3256571 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
ECDH1ME8569_RS16065 (ECDH1ME8569_3023) 3256820..3258100 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
ECDH1ME8569_RS16070 (ECDH1ME8569_3024) 3258123..3258596 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
ECDH1ME8569_RS16075 (ECDH1ME8569_3025) 3258607..3258978 + 372 Protein_3073 PTS sugar transporter subunit IIC -
ECDH1ME8569_RS16080 (ECDH1ME8569_3026) 3258974..3259531 + 558 Protein_3074 amidohydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3245760..3255707 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T27421 WP_000347273.1 NC_017638:3255243-3255707 [Escherichia coli DH1]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T27421 NC_017638:3255243-3255707 [Escherichia coli DH1]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT27421 WP_001307405.1 NC_017638:3254908-3255243 [Escherichia coli DH1]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT27421 NC_017638:3254908-3255243 [Escherichia coli DH1]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References