234515

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4086615..4087414 Replicon chromosome
Accession NZ_CP091682
Organism Escherichia coli strain KKo003

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag L6L54_RS19935 Protein ID WP_000347275.1
Coordinates 4086950..4087414 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L6L54_RS19930 Protein ID WP_001307405.1
Coordinates 4086615..4086950 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L54_RS19915 (4082400) 4082400..4083170 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
L6L54_RS19920 (4083186) 4083186..4084520 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L6L54_RS19925 (4084895) 4084895..4086466 + 1572 WP_001273752.1 galactarate dehydratase -
L6L54_RS19930 (4086615) 4086615..4086950 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6L54_RS19935 (4086950) 4086950..4087414 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6L54_RS19940 (4087469) 4087469..4088278 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L6L54_RS19945 (4088527) 4088527..4089807 + 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6L54_RS19950 (4089830) 4089830..4090303 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6L54_RS19955 (4090314) 4090314..4091093 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6L54_RS19960 (4091083) 4091083..4091961 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L6L54_RS19965 (4091979) 4091979..4092413 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4077467..4087414 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T234515 WP_000347275.1 NZ_CP091682:4086950-4087414 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T234515 NZ_LR130552:2069488-2069591 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT234515 WP_001307405.1 NZ_CP091682:4086615-4086950 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234515 NZ_LR130552:c2069629-2069358 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References