146264

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2347723..2348522 Replicon chromosome
Accession NZ_CP047461
Organism Escherichia coli strain ZF34

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag GTK64_RS11285 Protein ID WP_000347273.1
Coordinates 2348058..2348522 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GTK64_RS11280 Protein ID WP_001307405.1
Coordinates 2347723..2348058 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK64_RS11265 2343508..2344278 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
GTK64_RS11270 2344294..2345628 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GTK64_RS11275 2346003..2347574 + 1572 WP_001273753.1 galactarate dehydratase -
GTK64_RS11280 2347723..2348058 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GTK64_RS11285 2348058..2348522 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GTK64_RS11290 2348577..2349386 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GTK64_RS11295 2349635..2350915 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
GTK64_RS11300 2350938..2351411 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
GTK64_RS11305 2351422..2352201 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GTK64_RS11310 2352191..2353069 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GTK64_RS11315 2353087..2353521 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2338575..2348522 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T146264 WP_000347273.1 NZ_CP047461:2348058-2348522 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T146264 NZ_CP062733:c4706754-4706440 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGTAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT146264 WP_001307405.1 NZ_CP047461:2347723-2348058 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT146264 NZ_CP062733:c4706990-4706757 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References