137585

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 897927..898726 Replicon chromosome
Accession NZ_CP043195
Organism Escherichia coli O16:H48 strain PG20180062

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag FYA13_RS04315 Protein ID WP_000347273.1
Coordinates 897927..898391 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FYA13_RS04320 Protein ID WP_001307405.1
Coordinates 898391..898726 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA13_RS04290 894103..894660 - 558 Protein_822 amidohydrolase family protein -
FYA13_RS04295 894656..895027 - 372 Protein_823 PTS sugar transporter subunit IIC -
FYA13_RS04300 895038..895511 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
FYA13_RS04305 895534..896814 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
FYA13_RS04310 897063..897872 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FYA13_RS04315 897927..898391 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FYA13_RS04320 898391..898726 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FYA13_RS04325 898875..900446 - 1572 WP_001273753.1 galactarate dehydratase -
FYA13_RS04330 900821..902155 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
FYA13_RS04335 902171..902941 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 897927..909601 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T137585 WP_000347273.1 NZ_CP043195:c898391-897927 [Escherichia coli O16:H48]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T137585 NZ_CP043195:c898391-897927 [Escherichia coli O16:H48]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT137585 WP_001307405.1 NZ_CP043195:c898726-898391 [Escherichia coli O16:H48]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT137585 NZ_CP056834:c4062570-4062304 [Citrobacter sp. RHBSTW-00127]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGATGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGACT
TATTTAACTGGTTGATGAATCATGGGAAACCGGCTGATGCTCAGCTAGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References