110248

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 628504..629303 Replicon chromosome
Accession NZ_CP031833
Organism Escherichia coli strain 2452

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag D0R13_RS03235 Protein ID WP_000347273.1
Coordinates 628504..628968 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag D0R13_RS03240 Protein ID WP_001307405.1
Coordinates 628968..629303 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0R13_RS03200 623505..623939 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
D0R13_RS03205 623957..624835 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
D0R13_RS03210 624825..625604 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
D0R13_RS03215 625615..626088 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
D0R13_RS03220 626111..627391 - 1281 WP_117355235.1 tagatose-bisphosphate aldolase subunit KbaZ -
D0R13_RS03230 627640..628449 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
D0R13_RS03235 628504..628968 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
D0R13_RS03240 628968..629303 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
D0R13_RS03245 629452..631023 - 1572 WP_001273753.1 galactarate dehydratase -
D0R13_RS03250 631398..632732 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
D0R13_RS03255 632748..633518 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 628504..640178 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T110248 WP_000347273.1 NZ_CP031833:c628968-628504 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T110248 NZ_CP031833:c628968-628504 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT110248 WP_001307405.1 NZ_CP031833:c629303-628968 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT110248 NZ_CP031833:c629303-628968 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References