234262

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1980441..1981240 Replicon chromosome
Accession NZ_CP091652
Organism Escherichia coli strain KMi025

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag L6L63_RS09685 Protein ID WP_000347273.1
Coordinates 1980776..1981240 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L6L63_RS09680 Protein ID WP_001307405.1
Coordinates 1980441..1980776 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L63_RS09665 (1976226) 1976226..1976996 - 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -
L6L63_RS09670 (1977012) 1977012..1978346 - 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
L6L63_RS09675 (1978721) 1978721..1980292 + 1572 WP_001723936.1 galactarate dehydratase -
L6L63_RS09680 (1980441) 1980441..1980776 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6L63_RS09685 (1980776) 1980776..1981240 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6L63_RS09690 (1981295) 1981295..1982104 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L6L63_RS09695 (1982353) 1982353..1983633 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6L63_RS09700 (1983656) 1983656..1984129 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6L63_RS09705 (1984140) 1984140..1984919 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6L63_RS09710 (1984909) 1984909..1985787 + 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
L6L63_RS09715 (1985805) 1985805..1986239 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1971294..1981240 9946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T234262 WP_000347273.1 NZ_CP091652:1980776-1981240 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T234262 NZ_LR129841:1033432-1033686 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT234262 WP_001307405.1 NZ_CP091652:1980441-1980776 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234262 NZ_LR129841:1033188-1033430 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References