262372

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 642699..643498 Replicon chromosome
Accession NZ_CP109924
Organism Escherichia coli O125ac:K+:H10 strain C 236-04A

Toxin (Protein)


Gene name yhaV Uniprot ID D7XZ20
Locus tag N8A72_RS03120 Protein ID WP_000347264.1
Coordinates 642699..643163 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N8A72_RS03125 Protein ID WP_001307405.1
Coordinates 643163..643498 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A72_RS03090 (637700) 637700..638134 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N8A72_RS03095 (638152) 638152..639030 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
N8A72_RS03100 (639020) 639020..639799 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N8A72_RS03105 (639810) 639810..640283 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N8A72_RS03110 (640306) 640306..641586 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
N8A72_RS03115 (641835) 641835..642644 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N8A72_RS03120 (642699) 642699..643163 - 465 WP_000347264.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N8A72_RS03125 (643163) 643163..643498 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N8A72_RS03130 (643647) 643647..645218 - 1572 WP_001273753.1 galactarate dehydratase -
N8A72_RS03135 (645593) 645593..646927 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
N8A72_RS03140 (646943) 646943..647713 + 771 WP_001058207.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 642699..654268 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17894.29 Da        Isoelectric Point: 9.4945

>T262372 WP_000347264.1 NZ_CP109924:c643163-642699 [Escherichia coli O125ac:K+:H10]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFDAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT262372 WP_001307405.1 NZ_CP109924:c643498-643163 [Escherichia coli O125ac:K+:H10]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QML2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References