205539

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4063554..4064353 Replicon chromosome
Accession NZ_CP076344
Organism Escherichia coli strain AVS0096

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag KM937_RS19580 Protein ID WP_000347275.1
Coordinates 4063889..4064353 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KM937_RS19575 Protein ID WP_001307405.1
Coordinates 4063554..4063889 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM937_RS19560 (4059339) 4059339..4060109 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
KM937_RS19565 (4060125) 4060125..4061459 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KM937_RS19570 (4061834) 4061834..4063405 + 1572 WP_001273752.1 galactarate dehydratase -
KM937_RS19575 (4063554) 4063554..4063889 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KM937_RS19580 (4063889) 4063889..4064353 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KM937_RS19585 (4064408) 4064408..4065217 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KM937_RS19590 (4065466) 4065466..4066746 + 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
KM937_RS19595 (4066769) 4066769..4067242 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KM937_RS19600 (4067253) 4067253..4068032 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KM937_RS19605 (4068022) 4068022..4068900 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KM937_RS19610 (4068918) 4068918..4069352 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4054406..4064353 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T205539 WP_000347275.1 NZ_CP076344:4063889-4064353 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T205539 NZ_CP101726:877987-878394 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT205539 WP_001307405.1 NZ_CP076344:4063554-4063889 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT205539 NZ_CP101726:877740-878006 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References