263127

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 612156..612955 Replicon chromosome
Accession NZ_CP110117
Organism Escherichia coli strain 2-331

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag OMD12_RS03000 Protein ID WP_000347275.1
Coordinates 612156..612620 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag OMD12_RS03005 Protein ID WP_001307405.1
Coordinates 612620..612955 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OMD12_RS02970 (607157) 607157..607591 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
OMD12_RS02975 (607609) 607609..608487 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
OMD12_RS02980 (608477) 608477..609256 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
OMD12_RS02985 (609267) 609267..609740 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
OMD12_RS02990 (609763) 609763..611043 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
OMD12_RS02995 (611292) 611292..612101 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
OMD12_RS03000 (612156) 612156..612620 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
OMD12_RS03005 (612620) 612620..612955 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
OMD12_RS03010 (613104) 613104..614675 - 1572 WP_001273752.1 galactarate dehydratase -
OMD12_RS03015 (615050) 615050..616384 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
OMD12_RS03020 (616400) 616400..617170 + 771 WP_237998249.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 612156..623830 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T263127 WP_000347275.1 NZ_CP110117:c612620-612156 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT263127 WP_001307405.1 NZ_CP110117:c612955-612620 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References