140626

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2722918..2723717 Replicon chromosome
Accession NZ_CP044313
Organism Escherichia coli strain RM11911

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag F7D04_RS13685 Protein ID WP_000347251.1
Coordinates 2723253..2723717 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag F7D04_RS13680 Protein ID WP_001307405.1
Coordinates 2722918..2723253 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D04_RS13665 2718703..2719473 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
F7D04_RS13670 2719489..2720823 - 1335 WP_000599653.1 galactarate/glucarate/glycerate transporter GarP -
F7D04_RS13675 2721198..2722769 + 1572 WP_001273761.1 galactarate dehydratase -
F7D04_RS13680 2722918..2723253 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
F7D04_RS13685 2723253..2723717 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
F7D04_RS13690 2723772..2724581 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
F7D04_RS13695 2724830..2726110 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
F7D04_RS13700 2726133..2726606 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
F7D04_RS13705 2726617..2727396 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
F7D04_RS13710 2727386..2728264 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
F7D04_RS13715 2728282..2728716 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2713875..2723717 9842


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T140626 WP_000347251.1 NZ_CP044313:2723253-2723717 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T140626 NZ_CP059325:2261052-2261228 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT140626 WP_001307405.1 NZ_CP044313:2722918-2723253 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT140626 NZ_CP059325:2261282-2261665 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTTGGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References