Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4094238..4095037 Replicon chromosome
Accession NZ_CP029057
Organism Escherichia coli strain FORC_081

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag FORC81_RS20045 Protein ID WP_000347273.1
Coordinates 4094238..4094702 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FORC81_RS20050 Protein ID WP_001307405.1
Coordinates 4094702..4095037 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC81_RS20015 4089239..4089673 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
FORC81_RS20020 4089691..4090569 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
FORC81_RS20025 4090559..4091338 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
FORC81_RS20030 4091349..4091822 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
FORC81_RS20035 4091845..4093125 - 1281 WP_134162585.1 tagatose-bisphosphate aldolase subunit KbaZ -
FORC81_RS20040 4093374..4094183 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FORC81_RS20045 4094238..4094702 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FORC81_RS20050 4094702..4095037 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FORC81_RS20055 4095186..4096757 - 1572 WP_001273753.1 galactarate dehydratase -
FORC81_RS20060 4097132..4098466 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
FORC81_RS20065 4098482..4099252 + 771 WP_134162586.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4094238..4105912 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T104659 WP_000347273.1 NZ_CP029057:c4094702-4094238 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T104659 NZ_CP029057:c4094702-4094238 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT104659 WP_001307405.1 NZ_CP029057:c4095037-4094702 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT104659 NZ_CP029057:c4095037-4094702 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References