255693

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 639151..639950 Replicon chromosome
Accession NZ_CP103589
Organism Escherichia coli strain 4750

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag M5T00_RS03120 Protein ID WP_000347275.1
Coordinates 639151..639615 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5T00_RS03125 Protein ID WP_001307405.1
Coordinates 639615..639950 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T00_RS03090 (634152) 634152..634586 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5T00_RS03095 (634604) 634604..635482 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5T00_RS03100 (635472) 635472..636251 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5T00_RS03105 (636262) 636262..636735 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5T00_RS03110 (636758) 636758..638038 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5T00_RS03115 (638287) 638287..639096 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5T00_RS03120 (639151) 639151..639615 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5T00_RS03125 (639615) 639615..639950 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5T00_RS03130 (640099) 640099..641670 - 1572 WP_001273752.1 galactarate dehydratase -
M5T00_RS03135 (642045) 642045..643379 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
M5T00_RS03140 (643395) 643395..644165 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 639151..650825 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T255693 WP_000347275.1 NZ_CP103589:c639615-639151 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT255693 WP_001307405.1 NZ_CP103589:c639950-639615 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References