205729

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2473476..2474275 Replicon chromosome
Accession NZ_CP076470
Organism Escherichia coli strain DH5a

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KN807_RS11960 Protein ID WP_000347273.1
Coordinates 2473476..2473940 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KN807_RS11965 Protein ID WP_001307405.1
Coordinates 2473940..2474275 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KN807_RS11935 (2469652) 2469652..2470209 - 558 Protein_2322 amidohydrolase family protein -
KN807_RS11940 (2470205) 2470205..2470576 - 372 Protein_2323 PTS sugar transporter subunit IIC -
KN807_RS11945 (2470587) 2470587..2471060 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KN807_RS11950 (2471083) 2471083..2472363 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KN807_RS11955 (2472612) 2472612..2473421 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KN807_RS11960 (2473476) 2473476..2473940 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KN807_RS11965 (2473940) 2473940..2474275 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KN807_RS11970 (2474424) 2474424..2475995 - 1572 WP_001273747.1 galactarate dehydratase -
KN807_RS11975 (2476370) 2476370..2477704 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KN807_RS11980 (2477720) 2477720..2478490 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2473476..2485150 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T205729 WP_000347273.1 NZ_CP076470:c2473940-2473476 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T205729 NZ_CP101859:100510-100659 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT205729 WP_001307405.1 NZ_CP076470:c2474275-2473940 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT205729 NZ_CP101859:c100466-100405 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References