144228

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 637547..638346 Replicon chromosome
Accession NZ_CP046416
Organism Escherichia coli strain LZYWN_4

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag GJV83_RS03060 Protein ID WP_000347273.1
Coordinates 637547..638011 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GJV83_RS03065 Protein ID WP_001307405.1
Coordinates 638011..638346 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV83_RS03030 632548..632982 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
GJV83_RS03035 633000..633878 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GJV83_RS03040 633868..634647 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GJV83_RS03045 634658..635131 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
GJV83_RS03050 635154..636434 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
GJV83_RS03055 636683..637492 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GJV83_RS03060 637547..638011 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GJV83_RS03065 638011..638346 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GJV83_RS03070 638495..640066 - 1572 WP_001273753.1 galactarate dehydratase -
GJV83_RS03075 640441..641775 + 1335 WP_061093123.1 galactarate/glucarate/glycerate transporter GarP -
GJV83_RS03080 641791..642561 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 637547..649221 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T144228 WP_000347273.1 NZ_CP046416:c638011-637547 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T144228 NZ_CP061780:c1538973-1538695 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT144228 WP_001307405.1 NZ_CP046416:c638346-638011 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT144228 NZ_CP061780:c1538683-1538378 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References