59137

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3783722..3784521 Replicon chromosome
Accession NZ_CP013662
Organism Escherichia coli strain 08-00022

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag CP48_RS20580 Protein ID WP_000347273.1
Coordinates 3784057..3784521 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag CP48_RS20575 Protein ID WP_001307405.1
Coordinates 3783722..3784057 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP48_RS20560 3779507..3780277 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
CP48_RS20565 3780293..3781627 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
CP48_RS20570 3782002..3783573 + 1572 WP_001273753.1 galactarate dehydratase -
CP48_RS20575 3783722..3784057 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
CP48_RS20580 3784057..3784521 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
CP48_RS20585 3784576..3785385 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
CP48_RS20590 3785634..3786914 + 1281 WP_040090460.1 tagatose-bisphosphate aldolase subunit KbaZ -
CP48_RS20595 3786937..3787410 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
CP48_RS20600 3787421..3788200 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
CP48_RS20605 3788190..3789068 + 879 WP_040090458.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
CP48_RS20610 3789086..3789520 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3772952..3784521 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T59137 WP_000347273.1 NZ_CP013662:3784057-3784521 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T59137 NZ_CP013662:3784057-3784521 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT59137 WP_001307405.1 NZ_CP013662:3783722-3784057 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT59137 NZ_CP013662:3783722-3784057 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References