234848

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2344686..2345485 Replicon chromosome
Accession NZ_CP091836
Organism Escherichia coli strain SWYHX138.33gt

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag L7H17_RS11260 Protein ID WP_000347275.1
Coordinates 2345021..2345485 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L7H17_RS11255 Protein ID WP_001307405.1
Coordinates 2344686..2345021 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7H17_RS11240 (2340471) 2340471..2341241 - 771 WP_238058944.1 2-dehydro-3-deoxyglucarate aldolase -
L7H17_RS11245 (2341257) 2341257..2342591 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L7H17_RS11250 (2342966) 2342966..2344537 + 1572 WP_001273753.1 galactarate dehydratase -
L7H17_RS11255 (2344686) 2344686..2345021 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L7H17_RS11260 (2345021) 2345021..2345485 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L7H17_RS11265 (2345540) 2345540..2346349 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L7H17_RS11270 (2346598) 2346598..2347878 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
L7H17_RS11275 (2347901) 2347901..2348374 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
L7H17_RS11280 (2348385) 2348385..2349164 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
L7H17_RS11285 (2349154) 2349154..2350032 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L7H17_RS11290 (2350050) 2350050..2350484 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2335651..2345485 9834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T234848 WP_000347275.1 NZ_CP091836:2345021-2345485 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T234848 NZ_LR134092:2489011-2489298 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT234848 WP_001307405.1 NZ_CP091836:2344686-2345021 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234848 NZ_LR134092:2488772-2489011 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References