220279

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4026077..4026876 Replicon chromosome
Accession NZ_CP085060
Organism Escherichia coli strain P30_L1_05.20

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag LH369_RS19350 Protein ID WP_000347275.1
Coordinates 4026412..4026876 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LH369_RS19345 Protein ID WP_001307405.1
Coordinates 4026077..4026412 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH369_RS19330 (4021862) 4021862..4022632 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
LH369_RS19335 (4022648) 4022648..4023982 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LH369_RS19340 (4024357) 4024357..4025928 + 1572 WP_001273752.1 galactarate dehydratase -
LH369_RS19345 (4026077) 4026077..4026412 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LH369_RS19350 (4026412) 4026412..4026876 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LH369_RS19355 (4026931) 4026931..4027740 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LH369_RS19360 (4027989) 4027989..4029269 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LH369_RS19365 (4029292) 4029292..4029765 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LH369_RS19370 (4029776) 4029776..4030555 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
LH369_RS19375 (4030545) 4030545..4031423 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LH369_RS19380 (4031441) 4031441..4031875 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4016929..4026876 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T220279 WP_000347275.1 NZ_CP085060:4026412-4026876 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T220279 NZ_CP117001:c331673-331566 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT220279 WP_001307405.1 NZ_CP085060:4026077-4026412 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT220279 NZ_CP117001:331720-331786 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References