194799

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 297261..298060 Replicon chromosome
Accession NZ_CP070962
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JTR57_RS01525 Protein ID WP_000347273.1
Coordinates 297261..297725 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JTR57_RS01530 Protein ID WP_001307405.1
Coordinates 297725..298060 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS01500 (293437) 293437..293994 - 558 Protein_291 amidohydrolase family protein -
JTR57_RS01505 (293990) 293990..294361 - 372 Protein_292 PTS sugar transporter subunit IIC -
JTR57_RS01510 (294372) 294372..294845 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JTR57_RS01515 (294868) 294868..296148 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JTR57_RS01520 (296397) 296397..297206 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JTR57_RS01525 (297261) 297261..297725 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JTR57_RS01530 (297725) 297725..298060 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JTR57_RS01535 (298209) 298209..299780 - 1572 WP_001273753.1 galactarate dehydratase -
JTR57_RS01540 (300155) 300155..301489 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JTR57_RS01545 (301505) 301505..302275 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 297261..308935 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T194799 WP_000347273.1 NZ_CP070962:c297725-297261 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T194799 NZ_CP094076:614290-614562 [Helicobacter pylori]
ATGCTGATGATTGAAACCAGTAAAAAATTTGATAAGGATCTTAAAATTCTTGTTAAAAACGGGTTTGATTTAAAGCTTTT
ATATAAAGTGGTTGAAAATTTAGCCAAAGAGCAACCCCTAGATCCCAAATACAAAGACCACCCGCTCAAAGGTGCTTTAA
AAGATTTTAGGGAATGCCATCTAAAACCGGATTTATTGCTTGTCTATCAAATTAAAAAACAAGAAAACACCCTCTTTTTA
GTAAGACTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT194799 WP_001307405.1 NZ_CP070962:c298060-297725 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT194799 NZ_CP094076:613992-614303 [Helicobacter pylori]
ATGCCTAATTCTCCCACCAAAAAAGACTACACACGATACAGCGAAAAACAGCTTTTTAACTTTTTAAATTCTATCAAGAC
CAAACAAAAAAGAGCGTTAGAAAAATTGAAAGAAATCCAAGCTCAAAAACAAAGAATCAAAAAAGCACTCCAATTTAAAG
CGCTCCATTTAACCGAAAACGGATACACCATAGAAGAAGAGCGAGAAATCTTAGCAAGGGCTAAAGACACCAAGAACCAC
CTTTGTTTTAAAAATATAGAGGATTTTAAAAAGCATTGTGAAAACTTAAGATTTAAAAATGCTGATGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References