54116

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3251960..3252759 Replicon chromosome
Accession NZ_CP011343
Organism Escherichia coli K-12 substr. GM4792

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag U069_RS16065 Protein ID WP_000347273.1
Coordinates 3252295..3252759 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag U069_RS16060 Protein ID WP_001307405.1
Coordinates 3251960..3252295 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U069_RS16045 3247745..3248515 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
U069_RS16050 3248531..3249865 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
U069_RS16055 3250240..3251811 + 1572 WP_001273753.1 galactarate dehydratase -
U069_RS16060 3251960..3252295 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
U069_RS16065 3252295..3252759 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
U069_RS16070 3252814..3253623 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
U069_RS16075 3253872..3255152 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
U069_RS16080 3255175..3255648 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
U069_RS16085 3255659..3256030 + 372 Protein_3086 PTS sugar transporter subunit IIC -
U069_RS16090 3256026..3256583 + 558 Protein_3087 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3242925..3252759 9834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T54116 WP_000347273.1 NZ_CP011343:3252295-3252759 [Escherichia coli K-12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T54116 NZ_CP011343:3252295-3252759 [Escherichia coli K-12]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT54116 WP_001307405.1 NZ_CP011343:3251960-3252295 [Escherichia coli K-12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT54116 NZ_CP011343:3251960-3252295 [Escherichia coli K-12]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References