53422

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3233719..3234518 Replicon chromosome
Accession NZ_CP011113
Organism Escherichia coli strain RR1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag SR35_RS17120 Protein ID WP_000347273.1
Coordinates 3234054..3234518 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag SR35_RS17115 Protein ID WP_001307405.1
Coordinates 3233719..3234054 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SR35_RS17100 3229504..3230274 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
SR35_RS17105 3230290..3231624 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
SR35_RS17110 3231999..3233570 + 1572 WP_001273753.1 galactarate dehydratase -
SR35_RS17115 3233719..3234054 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
SR35_RS17120 3234054..3234518 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
SR35_RS17125 3234573..3235382 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
SR35_RS17135 3235631..3236911 + 1281 WP_001698595.1 tagatose-bisphosphate aldolase subunit KbaZ -
SR35_RS17140 3236934..3237407 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
SR35_RS17145 3237418..3237789 + 372 Protein_3063 PTS sugar transporter subunit IIC -
SR35_RS17150 3237785..3238342 + 558 Protein_3064 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3224571..3234518 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T53422 WP_000347273.1 NZ_CP011113:3234054-3234518 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T53422 NZ_CP011113:3234054-3234518 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT53422 WP_001307405.1 NZ_CP011113:3233719-3234054 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT53422 NZ_CP011113:3233719-3234054 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References