193574

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 609546..610345 Replicon chromosome
Accession NZ_CP070232
Organism Escherichia coli strain 10R

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag JTF63_RS02910 Protein ID WP_000347275.1
Coordinates 609546..610010 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JTF63_RS02915 Protein ID WP_001307405.1
Coordinates 610010..610345 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTF63_RS02885 605128..605460 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
JTF63_RS02890 605507..606382 - 876 WP_000239590.1 class A extended-spectrum beta-lactamase CTX-M-15 -
JTF63_RS02895 606638..607900 - 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
JTF63_RS02900 608086..608433 - 348 Protein_567 class II D-tagatose-bisphosphate aldolase, non-catalytic subunit -
JTF63_RS02905 608682..609491 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JTF63_RS02910 609546..610010 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JTF63_RS02915 610010..610345 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JTF63_RS02920 610494..612065 - 1572 WP_001273752.1 galactarate dehydratase -
JTF63_RS02925 612440..613774 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JTF63_RS02930 613790..614560 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 606535..621220 14685
- flank IS/Tn blaCTX-M-15 - 605507..607900 2393


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T193574 WP_000347275.1 NZ_CP070232:c610010-609546 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T193574 NZ_CP093031:c147651-147370 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT193574 WP_001307405.1 NZ_CP070232:c610345-610010 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT193574 NZ_CP093031:c147875-147648 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGATGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References