285670

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3273514..3274313 Replicon chromosome
Accession NZ_LN832404
Organism Escherichia coli K-12 substr. AG100

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag EK12AG100_RS16250 Protein ID WP_000347273.1
Coordinates 3273849..3274313 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EK12AG100_RS16245 Protein ID WP_001307405.1
Coordinates 3273514..3273849 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EK12AG100_RS16230 3269299..3270069 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
EK12AG100_RS16235 3270085..3271419 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
EK12AG100_RS16240 3271794..3273365 + 1572 WP_001273747.1 galactarate dehydratase -
EK12AG100_RS16245 3273514..3273849 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EK12AG100_RS16250 3273849..3274313 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EK12AG100_RS16255 3274368..3275177 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
EK12AG100_RS16260 3275426..3276706 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
EK12AG100_RS16265 3276729..3277202 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
EK12AG100_RS16270 3277213..3277584 + 372 Protein_3101 PTS sugar transporter subunit IIC -
EK12AG100_RS16275 3277580..3278137 + 558 Protein_3102 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3264366..3274313 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T285670 WP_000347273.1 NZ_LN832404:3273849-3274313 [Escherichia coli K-12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT285670 WP_001307405.1 NZ_LN832404:3273514-3273849 [Escherichia coli K-12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References