128270

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611446..612245 Replicon chromosome
Accession NZ_CP041392
Organism Escherichia coli strain ECOL-18-VL-LA-PA-Ryan-0026

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag E3Z24_RS02975 Protein ID WP_000347275.1
Coordinates 611446..611910 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag E3Z24_RS02980 Protein ID WP_001307405.1
Coordinates 611910..612245 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3Z24_RS02945 606447..606881 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
E3Z24_RS02950 606899..607777 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
E3Z24_RS02955 607767..608546 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
E3Z24_RS02960 608557..609030 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
E3Z24_RS02965 609053..610333 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
E3Z24_RS02970 610582..611391 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
E3Z24_RS02975 611446..611910 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
E3Z24_RS02980 611910..612245 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
E3Z24_RS02985 612394..613965 - 1572 WP_001273752.1 galactarate dehydratase -
E3Z24_RS02990 614340..615674 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
E3Z24_RS02995 615690..616460 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 611446..623120 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T128270 WP_000347275.1 NZ_CP041392:c611910-611446 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T128270 NZ_CP041392:c611910-611446 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT128270 WP_001307405.1 NZ_CP041392:c612245-611910 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT128270 NZ_CP041392:c612245-611910 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References