64593

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4018980..4019779 Replicon chromosome
Accession NZ_CP016404
Organism Escherichia coli strain 210221272

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag BBP24_RS19885 Protein ID WP_000347273.1
Coordinates 4019315..4019779 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag BBP24_RS19880 Protein ID WP_001307405.1
Coordinates 4018980..4019315 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBP24_RS19865 4014765..4015535 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
BBP24_RS19870 4015551..4016885 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
BBP24_RS19875 4017260..4018831 + 1572 WP_001273753.1 galactarate dehydratase -
BBP24_RS19880 4018980..4019315 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
BBP24_RS19885 4019315..4019779 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
BBP24_RS19890 4019834..4020643 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
BBP24_RS19895 4020892..4022172 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
BBP24_RS19900 4022195..4022668 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
BBP24_RS19905 4022679..4023458 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
BBP24_RS19910 4023448..4024326 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
BBP24_RS19915 4024344..4024778 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4009832..4019779 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T64593 WP_000347273.1 NZ_CP016404:4019315-4019779 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T64593 NZ_CP016404:4019315-4019779 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT64593 WP_001307405.1 NZ_CP016404:4018980-4019315 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT64593 NZ_CP016404:4018980-4019315 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References