140535

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1671439..1672238 Replicon chromosome
Accession NZ_CP044293
Organism Escherichia coli strain P276M

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag F6P95_RS08195 Protein ID WP_000347273.1
Coordinates 1671774..1672238 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag F6P95_RS08190 Protein ID WP_001307405.1
Coordinates 1671439..1671774 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6P95_RS08175 1667232..1668002 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
F6P95_RS08180 1668018..1669352 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
F6P95_RS08185 1669719..1671290 + 1572 WP_072778057.1 galactarate dehydratase -
F6P95_RS08190 1671439..1671774 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
F6P95_RS08195 1671774..1672238 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
F6P95_RS08200 1672293..1673102 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
F6P95_RS08205 1673351..1674631 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
F6P95_RS08210 1674654..1675127 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
F6P95_RS08215 1675138..1675917 + 780 WP_000406208.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
F6P95_RS08220 1675907..1676785 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
F6P95_RS08225 1676803..1677237 + 435 WP_000948842.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1662444..1672238 9794


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T140535 WP_000347273.1 NZ_CP044293:1671774-1672238 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T140535 NZ_CP059295:2146136-2146546 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT140535 WP_001307405.1 NZ_CP044293:1671439-1671774 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT140535 NZ_CP059295:2145889-2146155 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References