103806

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4126515..4127314 Replicon chromosome
Accession NZ_CP028760
Organism Escherichia coli strain A14

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag AOY67_RS20070 Protein ID WP_000347273.1
Coordinates 4126850..4127314 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag AOY67_RS20065 Protein ID WP_001307405.1
Coordinates 4126515..4126850 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY67_RS20050 (4122300) 4122300..4123070 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
AOY67_RS20055 (4123086) 4123086..4124420 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
AOY67_RS20060 (4124795) 4124795..4126366 + 1572 WP_001273753.1 galactarate dehydratase -
AOY67_RS20065 (4126515) 4126515..4126850 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AOY67_RS20070 (4126850) 4126850..4127314 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AOY67_RS20075 (4127369) 4127369..4128178 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AOY67_RS20080 (4128427) 4128427..4129707 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
AOY67_RS20085 (4129730) 4129730..4130203 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
AOY67_RS20090 (4130214) 4130214..4130993 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
AOY67_RS20095 (4130983) 4130983..4131861 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
AOY67_RS20100 (4131879) 4131879..4132313 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4117367..4127314 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T103806 WP_000347273.1 NZ_CP028760:4126850-4127314 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T103806 NZ_CP028760:4126850-4127314 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT103806 WP_001307405.1 NZ_CP028760:4126515-4126850 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT103806 NZ_CP028760:4126515-4126850 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References