Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3283077..3283876 Replicon chromosome
Accession NZ_CP084899
Organism Escherichia coli strain W3110-P

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LHV60_RS16025 Protein ID WP_000347273.1
Coordinates 3283412..3283876 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LHV60_RS16020 Protein ID WP_001307405.1
Coordinates 3283077..3283412 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV60_RS16005 (3278862) 3278862..3279632 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
LHV60_RS16010 (3279648) 3279648..3280982 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LHV60_RS16015 (3281357) 3281357..3282928 + 1572 WP_001273753.1 galactarate dehydratase -
LHV60_RS16020 (3283077) 3283077..3283412 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LHV60_RS16025 (3283412) 3283412..3283876 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LHV60_RS16030 (3283931) 3283931..3284740 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LHV60_RS16035 (3284989) 3284989..3286269 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LHV60_RS16040 (3286292) 3286292..3286765 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LHV60_RS16045 (3286776) 3286776..3287147 + 372 Protein_3134 PTS sugar transporter subunit IIC -
LHV60_RS16050 (3287143) 3287143..3287700 + 558 Protein_3135 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3273929..3283876 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T220102 WP_000347273.1 NZ_CP084899:3283412-3283876 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T220102 NZ_CP116931:c633105-632641 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT220102 WP_001307405.1 NZ_CP084899:3283077-3283412 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT220102 NZ_CP116931:c633440-633105 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References