201519

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3662932..3663731 Replicon chromosome
Accession NZ_CP074120
Organism Escherichia coli strain EC16

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag KGQ53_RS17515 Protein ID WP_000347275.1
Coordinates 3662932..3663396 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KGQ53_RS17520 Protein ID WP_001307405.1
Coordinates 3663396..3663731 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGQ53_RS17485 (3657933) 3657933..3658367 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KGQ53_RS17490 (3658385) 3658385..3659263 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KGQ53_RS17495 (3659253) 3659253..3660032 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KGQ53_RS17500 (3660043) 3660043..3660516 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KGQ53_RS17505 (3660539) 3660539..3661819 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KGQ53_RS17510 (3662068) 3662068..3662877 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KGQ53_RS17515 (3662932) 3662932..3663396 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KGQ53_RS17520 (3663396) 3663396..3663731 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KGQ53_RS17525 (3663880) 3663880..3665451 - 1572 WP_001273752.1 galactarate dehydratase -
KGQ53_RS17530 (3665826) 3665826..3667160 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KGQ53_RS17535 (3667176) 3667176..3667946 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3662932..3674606 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T201519 WP_000347275.1 NZ_CP074120:c3663396-3662932 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T201519 NZ_CP098479:447743-448132 [Acinetobacter sp. C26G]
ATGGCTAATGGTGTTTTCGAGCTGCAACGCAGTCGTTTTGCGATGGTATTGCAGCTCTTGATTTTCATTGCCATCCTTAG
TTTAACTTATTCCTTGCTTGCCTTATGGATTTGGCTATTGTGCTTCTGCATGATGGGAGCTGCTTGGATTTTGTTTCTCA
AACAGCCACAGATTAAACGTTTTGAATACTTGGATCATCAAGAATGTTCGTTCGAGTTTTATGACCCCGAATTAAAGATT
CAGCGCAGAAAAATCGTTAAAATATTGGATCACAAATTTTACATCGCACTCTATTTTTCTCATTCAAACCAGAGAACTTG
TATCATTTGGTGGGATCAATTATCACATTCTCAGTGGCAAAAGCTAAAATTACGCGCAAAATTGGCATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT201519 WP_001307405.1 NZ_CP074120:c3663731-3663396 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT201519 NZ_CP098479:447493-447750 [Acinetobacter sp. C26G]
ATGTCTGATGAAATGACACTGGAAGAGCGTAAGGTTGTGTATCGTGCACGTCGTGGTTTAAAAGAAATCGATGTGTATTT
TGATCCTTATGTTAAAAACCACTATCTGACAGCTGATATCGCTGAAAAGGCATTATTTGCGGAGTTGGTCGATCAAGAAG
ATCCTGATTTATTGGATTGGTTTATGGAGGTTGGAGAACCGCCTCGTGCAGAATTAAAAGATTTTATTTTAAAATTAAAA
CACTATGTCCATGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References