55857

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3254911..3255710 Replicon chromosome
Accession NZ_CP012125
Organism Escherichia coli strain DH1Ec095

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag ADS71_RS16025 Protein ID WP_000347273.1
Coordinates 3255246..3255710 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag ADS71_RS16020 Protein ID WP_001307405.1
Coordinates 3254911..3255246 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADS71_RS16005 3250696..3251466 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
ADS71_RS16010 3251482..3252816 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
ADS71_RS16015 3253191..3254762 + 1572 WP_001273747.1 galactarate dehydratase -
ADS71_RS16020 3254911..3255246 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
ADS71_RS16025 3255246..3255710 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
ADS71_RS16030 3255765..3256574 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
ADS71_RS16035 3256823..3258103 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
ADS71_RS16040 3258126..3258599 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
ADS71_RS16045 3258610..3258981 + 372 Protein_3073 PTS sugar transporter subunit IIC -
ADS71_RS16050 3258977..3259534 + 558 Protein_3074 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3245763..3255710 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T55857 WP_000347273.1 NZ_CP012125:3255246-3255710 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T55857 NZ_CP012125:3255246-3255710 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT55857 WP_001307405.1 NZ_CP012125:3254911-3255246 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT55857 NZ_CP012125:3254911-3255246 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References