199025

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 607831..608630 Replicon chromosome
Accession NZ_CP072858
Organism Escherichia coli strain 184/2aE

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag J9158_RS02955 Protein ID WP_000347275.1
Coordinates 607831..608295 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J9158_RS02960 Protein ID WP_001307405.1
Coordinates 608295..608630 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9158_RS02925 (602832) 602832..603266 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
J9158_RS02930 (603284) 603284..604162 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
J9158_RS02935 (604152) 604152..604931 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
J9158_RS02940 (604942) 604942..605415 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
J9158_RS02945 (605438) 605438..606718 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
J9158_RS02950 (606967) 606967..607776 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J9158_RS02955 (607831) 607831..608295 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J9158_RS02960 (608295) 608295..608630 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J9158_RS02965 (608779) 608779..610350 - 1572 WP_001273752.1 galactarate dehydratase -
J9158_RS02970 (610725) 610725..612059 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
J9158_RS02975 (612075) 612075..612845 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 607831..619505 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T199025 WP_000347275.1 NZ_CP072858:c608295-607831 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T199025 NZ_CP097107:4265250-4265630 [Citrobacter freundii]
ATGCAGACCTTATCCGCCATCCCGACGCGGAAGGCACCGTCACACCCGACGCCCGTTGAAATCTGGCAGCAACTTCTTAC
GTATCTGTTAAAAAGACATTACGGCCTTTCGCTTAGCGACACGCAATTTTCCGATGAGAAGATTATTACGCAATATATTG
ACGCCGGTATCTCACTGTCAGATGCACTCAATTTTCTCGTTGAAAAATCTGAACTGGTGCGGATTGATCGTCCCGGTTTA
TCCATTAAGCATCAGTCCCCTTTTATCGGGGTGATTGATATTCTCCGGGCCAGACGGGCAACGGGTTTGATGCAACGTAA
CGGGTATAAAAGGATCACTCTTCTCATCGCTGGAAATGCAGCTCAGGAGCAACATTCATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT199025 WP_001307405.1 NZ_CP072858:c608630-608295 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT199025 NZ_CP097107:4264827-4265192 [Citrobacter freundii]
TTGTCGAATCAACTACCTCCAGTAAACCATGATGTCTCTGAGCCCTGGTGGGGGCTGAAACCGGGCATCACTCCGTGTTT
TGGCGCACGACTGGTACAGGAAGGCAACCGCCTGCATTATCTGGCTGATCGGGCCAGTATCGCCGGTGTGTTCAGCGATG
CGGATTTACGCCATCTCGATCAGGCATTTCCGGCACTGCTCAAACAGCTGGAATTAATGCTGGTTTCCGGTGAGCTCAAT
CCCCGCCATCAGCACTGTGTCACGTTATATGCAAAAGGGCTGACCTGTGAAGCAGATTCCCTCGGATCACATGGCTACAT
TTATACAGCAATGTATCCGACACCAGACAACAGCATAACCCGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References