211037

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3237151..3237950 Replicon chromosome
Accession NZ_CP080139
Organism Escherichia coli strain PK8241

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KZY05_RS16510 Protein ID WP_000347273.1
Coordinates 3237486..3237950 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0K5UM65
Locus tag KZY05_RS16505 Protein ID WP_025670440.1
Coordinates 3237151..3237486 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY05_RS16490 (3232936) 3232936..3233706 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
KZY05_RS16495 (3233722) 3233722..3235056 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KZY05_RS16500 (3235431) 3235431..3237002 + 1572 WP_032202412.1 galactarate dehydratase -
KZY05_RS16505 (3237151) 3237151..3237486 + 336 WP_025670440.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZY05_RS16510 (3237486) 3237486..3237950 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZY05_RS16515 (3238005) 3238005..3238814 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZY05_RS16520 (3239063) 3239063..3240343 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZY05_RS16525 (3240366) 3240366..3240839 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZY05_RS16530 (3240850) 3240850..3241629 + 780 WP_032216390.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZY05_RS16535 (3241619) 3241619..3242497 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZY05_RS16540 (3242515) 3242515..3242949 + 435 WP_000948814.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3228003..3237950 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T211037 WP_000347273.1 NZ_CP080139:3237486-3237950 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T211037 NZ_CP104665:c3972969-3972571 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12387.00 Da        Isoelectric Point: 5.1498

>AT211037 WP_025670440.1 NZ_CP080139:3237151-3237486 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PRKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211037 NZ_CP104665:c3973265-3972972 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0K5UM65

References