168020

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3713154..3713953 Replicon chromosome
Accession NZ_CP058948
Organism Escherichia coli strain SY3626C5

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HZ988_RS17975 Protein ID WP_000347273.1
Coordinates 3713489..3713953 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZ988_RS17970 Protein ID WP_001307405.1
Coordinates 3713154..3713489 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZ988_RS17955 3708939..3709709 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
HZ988_RS17960 3709725..3711059 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HZ988_RS17965 3711434..3713005 + 1572 WP_001273753.1 galactarate dehydratase -
HZ988_RS17970 3713154..3713489 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZ988_RS17975 3713489..3713953 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZ988_RS17980 3714008..3714817 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZ988_RS17985 3715066..3716346 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZ988_RS17990 3716369..3716842 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZ988_RS17995 3716853..3717224 + 372 Protein_3520 PTS sugar transporter subunit IIC -
HZ988_RS18000 3717220..3717777 + 558 Protein_3521 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3704006..3713953 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T168020 WP_000347273.1 NZ_CP058948:3713489-3713953 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T168020 NZ_CP075566:5943391-5943573 [Pseudomonas fluorescens]
ATGCGAAGTCGGGAAATGATCAGGATGATCGAGGAGGATGGTTGGTATTTGGTCGCCGTGAAAGATAGCCACCATCAGTA
CAAGCATTCGTACAAGGCAGGCCGGGTGACGATCAAGCACCCTGATTCGGATCTGCCCAAAGGAACGATCAACAGCATTT
TGAAACAGGCGGGTCTGAAATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT168020 WP_001307405.1 NZ_CP058948:3713154-3713489 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT168020 NZ_CP075566:5943570-5944034 [Pseudomonas fluorescens]
ATGACGCCCGACATTGGAAACGAGAGCCTCGAAGGGGCCACAGCACAGGAAGGGCTTACCGAAATGAAATTCCCGGTCGT
TCTGCACAAGGATGCCGACTCAGAATACGGAGTGATTATCCCGGACGTACCGGGTTGTTTCTCTGCCGGCGCTACAGTGG
CGCAGGCATTCGAAAACGTGAAGGAAGCGCTTTCCTTGCACTATGAAGGGTTGGTCGCCGATGGTGATCCACTGCCGCAA
GTGCGTGAGATTGACGCTCATATTGATAACCCTGATTACGCCGGTGGTGTGTGGGGGGTGGTCGACTTCGATATCACGCC
TTACTTCGGCAAGTCGGTGCGCTTCAACGCCACGCTGCCTGAGCAGTTGCTTGAGCGTATCGACCAAACGGTCAGGCGGG
ATCAGCGATACAGCTCAAGATCCGGTTTTTTGGCTGCGGCCGCATTGCGTGAGCTGTCGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References