136540

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 625883..626682 Replicon chromosome
Accession NZ_CP042901
Organism Escherichia coli strain CFSAN061762

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag CA898_RS03010 Protein ID WP_000347273.1
Coordinates 625883..626347 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag CA898_RS03015 Protein ID WP_001307405.1
Coordinates 626347..626682 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA898_RS02980 (620884) 620884..621318 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
CA898_RS02985 (621336) 621336..622214 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
CA898_RS02990 (622204) 622204..622983 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
CA898_RS02995 (622994) 622994..623467 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
CA898_RS03000 (623490) 623490..624770 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
CA898_RS03005 (625019) 625019..625828 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
CA898_RS03010 (625883) 625883..626347 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
CA898_RS03015 (626347) 626347..626682 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
CA898_RS03020 (626831) 626831..628402 - 1572 WP_001273753.1 galactarate dehydratase -
CA898_RS03025 (628777) 628777..630111 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
CA898_RS03030 (630127) 630127..630897 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 625883..637557 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T136540 WP_000347273.1 NZ_CP042901:c626347-625883 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T136540 NZ_CP042901:c626347-625883 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT136540 WP_001307405.1 NZ_CP042901:c626682-626347 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT136540 NZ_CP056129:46656-46922 [Enterobacter roggenkampii]
ATGGAACGCACAGCAAAACTATTCAAAAAGGGTCAGAATCAGGCCGTTATGCTGCCTGCTGAATTCGCGTTTGATACGGA
GAGCGTCTACATCCGACGGGATGAGGAGGGGAATGTCGTTTTGGCGGCAAAACCTGTTCTAATAAAGAGCTGGGATAATT
TTCTCCGCATGCTGGCAGAAACGGACGTACCAGATACGTTTTTGAGCAAGGAGGAACGCAATCAGAGTGTTACGACAAGA
GACCCTCTGGATGGAGCATGCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References