169102

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2386277..2387076 Replicon chromosome
Accession NZ_CP059723
Organism Escherichia coli strain BUCT2930

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H1R14_RS13345 Protein ID WP_000347273.1
Coordinates 2386612..2387076 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H1R14_RS13340 Protein ID WP_001307405.1
Coordinates 2386277..2386612 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R14_RS13325 2382062..2382832 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
H1R14_RS13330 2382848..2384182 - 1335 WP_061093123.1 galactarate/glucarate/glycerate transporter GarP -
H1R14_RS13335 2384557..2386128 + 1572 WP_001273753.1 galactarate dehydratase -
H1R14_RS13340 2386277..2386612 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H1R14_RS13345 2386612..2387076 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H1R14_RS13350 2387131..2387940 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H1R14_RS13355 2388189..2389469 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H1R14_RS13360 2389492..2389965 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H1R14_RS13365 2389976..2390755 + 780 WP_112929341.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H1R14_RS13370 2390745..2391623 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H1R14_RS13375 2391641..2392075 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2377129..2387076 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T169102 WP_000347273.1 NZ_CP059723:2386612-2387076 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T169102 NZ_CP076277:c39432-39283 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTTACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT169102 WP_001307405.1 NZ_CP059723:2386277-2386612 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT169102 NZ_CP076277:39476-39537 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References