256441

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4316930..4317729 Replicon chromosome
Accession NZ_CP103739
Organism Escherichia coli strain 3359

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag M5T33_RS21275 Protein ID WP_000347273.1
Coordinates 4316930..4317394 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5T33_RS21280 Protein ID WP_001307405.1
Coordinates 4317394..4317729 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T33_RS21245 (4312359) 4312359..4313348 - 990 WP_000548347.1 bifunctional threonine ammonia-lyase/L-serine ammonia-lyase TdcB -
M5T33_RS21250 (4313447) 4313447..4314385 - 939 WP_000104211.1 transcriptional regulator TdcA -
M5T33_RS21255 (4314700) 4314700..4314918 + 219 WP_001301311.1 transcriptional activator TdcR -
M5T33_RS21260 (4315174) 4315174..4315713 + 540 WP_000675733.1 protein YhaB -
M5T33_RS21265 (4315735) 4315735..4316097 + 363 Protein_4146 hypothetical protein -
M5T33_RS21275 (4316930) 4316930..4317394 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5T33_RS21280 (4317394) 4317394..4317729 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5T33_RS21285 (4317878) 4317878..4319449 - 1572 WP_001273753.1 galactarate dehydratase -
M5T33_RS21290 (4319824) 4319824..4321158 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
M5T33_RS21295 (4321174) 4321174..4321944 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4315174..4326493 11319
- flank IS/Tn - - 4316114..4316491 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T256441 WP_000347273.1 NZ_CP103739:c4317394-4316930 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT256441 WP_001307405.1 NZ_CP103739:c4317729-4317394 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References