145450

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2475819..2476618 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag GRF62_RS11840 Protein ID WP_000347273.1
Coordinates 2475819..2476283 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GRF62_RS11845 Protein ID WP_001307405.1
Coordinates 2476283..2476618 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS11815 2471995..2472552 - 558 Protein_2289 amidohydrolase family protein -
GRF62_RS11820 2472548..2472919 - 372 Protein_2290 PTS sugar transporter subunit IIC -
GRF62_RS11825 2472930..2473403 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
GRF62_RS11830 2473426..2474706 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
GRF62_RS11835 2474955..2475764 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GRF62_RS11840 2475819..2476283 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GRF62_RS11845 2476283..2476618 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GRF62_RS11850 2476767..2478338 - 1572 WP_001273747.1 galactarate dehydratase -
GRF62_RS11855 2478713..2480047 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GRF62_RS11860 2480063..2480833 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2475819..2487492 11673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T145450 WP_000347273.1 NZ_CP047127:c2476283-2475819 [Escherichia coli K-12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T145450 NZ_CP062386:2279982-2280248 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT145450 WP_001307405.1 NZ_CP047127:c2476618-2476283 [Escherichia coli K-12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT145450 NZ_CP062386:2279725-2279982 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References