177112

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 617672..618471 Replicon chromosome
Accession NZ_CP062883
Organism Escherichia coli strain Res13-Lact-PEA06-10

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag IMP66_RS02990 Protein ID WP_000347273.1
Coordinates 617672..618136 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IMP66_RS02995 Protein ID WP_001307405.1
Coordinates 618136..618471 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP66_RS02960 612673..613107 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IMP66_RS02965 613125..614003 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IMP66_RS02970 613993..614772 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IMP66_RS02975 614783..615256 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
IMP66_RS02980 615279..616559 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
IMP66_RS02985 616808..617617 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IMP66_RS02990 617672..618136 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IMP66_RS02995 618136..618471 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IMP66_RS03000 618620..620191 - 1572 WP_001273753.1 galactarate dehydratase -
IMP66_RS03005 620566..621900 + 1335 WP_095885843.1 galactarate/glucarate/glycerate transporter GarP -
IMP66_RS03010 621916..622686 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 617672..629346 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T177112 WP_000347273.1 NZ_CP062883:c618136-617672 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T177112 NZ_CP083144:5665671-5666021 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT177112 WP_001307405.1 NZ_CP062883:c618471-618136 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT177112 NZ_CP083144:5665379-5665666 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References