149216

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 649946..650745 Replicon chromosome
Accession NZ_CP048367
Organism Escherichia coli strain 164

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag GZS07_RS03115 Protein ID WP_000347275.1
Coordinates 649946..650410 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GZS07_RS03120 Protein ID WP_001307405.1
Coordinates 650410..650745 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS07_RS03085 644947..645381 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
GZS07_RS03090 645399..646277 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GZS07_RS03095 646267..647046 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GZS07_RS03100 647057..647530 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
GZS07_RS03105 647553..648833 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
GZS07_RS03110 649082..649891 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GZS07_RS03115 649946..650410 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GZS07_RS03120 650410..650745 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GZS07_RS03125 650894..652465 - 1572 WP_001273752.1 galactarate dehydratase -
GZS07_RS03130 652840..654174 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GZS07_RS03135 654190..654960 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 649946..661620 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T149216 WP_000347275.1 NZ_CP048367:c650410-649946 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T149216 NZ_CP063945:5011161-5011646 [Klebsiella pneumoniae]
ATGATCTTGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT149216 WP_001307405.1 NZ_CP048367:c650745-650410 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT149216 NZ_CP063945:5010871-5011164 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAACAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References