186384

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611185..611984 Replicon chromosome
Accession NZ_CP067994
Organism Escherichia coli strain CV601

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JJT18_RS02950 Protein ID WP_000347273.1
Coordinates 611185..611649 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJT18_RS02955 Protein ID WP_001307405.1
Coordinates 611649..611984 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJT18_RS02925 607361..607918 - 558 Protein_572 amidohydrolase family protein -
JJT18_RS02930 607914..608285 - 372 Protein_573 PTS sugar transporter subunit IIC -
JJT18_RS02935 608296..608769 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJT18_RS02940 608792..610072 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJT18_RS02945 610321..611130 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JJT18_RS02950 611185..611649 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJT18_RS02955 611649..611984 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJT18_RS02960 612133..613704 - 1572 WP_001273753.1 galactarate dehydratase -
JJT18_RS02965 614079..615413 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JJT18_RS02970 615429..616199 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 611185..622857 11672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T186384 WP_000347273.1 NZ_CP067994:c611649-611185 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T186384 NZ_CP089407:809074-809177 [Klebsiella michiganensis]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT186384 WP_001307405.1 NZ_CP067994:c611984-611649 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT186384 NZ_CP089407:c809184-809016 [Klebsiella michiganensis]
AACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGCGACAAAAGTG
GACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References