249132

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 606625..607424 Replicon chromosome
Accession NZ_CP099588
Organism Escherichia coli strain EMG2

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag NHF38_RS02945 Protein ID WP_000347273.1
Coordinates 606625..607089 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NHF38_RS02950 Protein ID WP_001307405.1
Coordinates 607089..607424 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF38_RS02920 (602801) 602801..603358 - 558 Protein_576 amidohydrolase family protein -
NHF38_RS02925 (603354) 603354..603725 - 372 Protein_577 PTS sugar transporter subunit IIC -
NHF38_RS02930 (603736) 603736..604209 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
NHF38_RS02935 (604232) 604232..605512 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
NHF38_RS02940 (605761) 605761..606570 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NHF38_RS02945 (606625) 606625..607089 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NHF38_RS02950 (607089) 607089..607424 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NHF38_RS02955 (607573) 607573..609144 - 1572 WP_001273753.1 galactarate dehydratase -
NHF38_RS02960 (609519) 609519..610853 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NHF38_RS02965 (610869) 610869..611639 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 606625..618299 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T249132 WP_000347273.1 NZ_CP099588:c607089-606625 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT249132 WP_001307405.1 NZ_CP099588:c607424-607089 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References