210990

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 606490..607289 Replicon chromosome
Accession NZ_CP080133
Organism Escherichia coli strain PK8277

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KZY04_RS02960 Protein ID WP_000347273.1
Coordinates 606490..606954 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KZY04_RS02965 Protein ID WP_001307405.1
Coordinates 606954..607289 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY04_RS02930 (601491) 601491..601925 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZY04_RS02935 (601943) 601943..602821 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZY04_RS02940 (602811) 602811..603590 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZY04_RS02945 (603601) 603601..604074 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZY04_RS02950 (604097) 604097..605377 - 1281 WP_130568314.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZY04_RS02955 (605626) 605626..606435 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZY04_RS02960 (606490) 606490..606954 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZY04_RS02965 (606954) 606954..607289 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZY04_RS02970 (607438) 607438..609009 - 1572 WP_001273753.1 galactarate dehydratase -
KZY04_RS02975 (609384) 609384..610718 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KZY04_RS02980 (610734) 610734..611504 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 606490..618051 11561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T210990 WP_000347273.1 NZ_CP080133:c606954-606490 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T210990 NZ_CP104651:2739006-2739401 [Acinetobacter variabilis]
ATGATTTACCTCTTAGACACAAATATTTGTATCTACGTGATCAATCATAAACCACAGCAAGTTTTTGAACGCTTTAAGCA
ATATCAGCTTGGACAGTTAGCTATTTCTAGCATAACCGCGTCAGAGCTGGCTTTTGGCGTTGAAAAGTCTGGATCTGAAC
GCAATAAGCAAGCTCTGAACAAGTTTTTATCACCCTTAGATATTTTACCCTACGATGAGCAAGCGATTTGGCATTACGCT
AAATTACGTCAAGATCTCCAATCAACAGGTAAAACTATTGGTAGTTTAGATATGCTAATAGCAGCTCATGCCCTCGCTTT
AGATGTTGTACTAGTCACAAACAACATCAAGGAATTTGAGCGTATAGATGGGTTAAAGTTAGAGAACTGGACCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT210990 WP_001307405.1 NZ_CP080133:c607289-606954 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT210990 NZ_CP104651:2738779-2739009 [Acinetobacter variabilis]
ATGGAAACCGCTAAAGTTTTTCAAACAGGTCGTAGTCAAGCTGTCCGTTTACCTAAAGCATTTCGCTTTAATGGTACTGA
AGTTGCTATCAAGAGTTTTGGCCGTGGTGTCTTATTAATGCCCATCGAAAACCCATGGGACATCATGTTAGAAGCAATTA
ACGAATTTGAAGTAGGATTTCAGCTTGAACGAGCTGATCAGGGCGAGCAATTACGTGAGGACTTCAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References