200645

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 602150..602949 Replicon chromosome
Accession NZ_CP073936
Organism Escherichia coli strain MB56

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag KFU61_RS02930 Protein ID WP_000347275.1
Coordinates 602150..602614 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU61_RS02935 Protein ID WP_001307405.1
Coordinates 602614..602949 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU61_RS02900 (597151) 597151..597585 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU61_RS02905 (597603) 597603..598481 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU61_RS02910 (598471) 598471..599250 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU61_RS02915 (599261) 599261..599734 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU61_RS02920 (599757) 599757..601037 - 1281 WP_257181981.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU61_RS02925 (601286) 601286..602095 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU61_RS02930 (602150) 602150..602614 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU61_RS02935 (602614) 602614..602949 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU61_RS02940 (603098) 603098..604669 - 1572 WP_001273753.1 galactarate dehydratase -
KFU61_RS02945 (605044) 605044..606378 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU61_RS02950 (606394) 606394..607164 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 602150..613824 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T200645 WP_000347275.1 NZ_CP073936:c602614-602150 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T200645 NZ_CP098131:821598-821816 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200645 WP_001307405.1 NZ_CP073936:c602949-602614 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200645 NZ_CP098131:821197-821571 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References