97286

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3983431..3984230 Replicon chromosome
Accession NZ_CP027105
Organism Escherichia coli strain RM14721

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag C5097_RS20980 Protein ID WP_000347273.1
Coordinates 3983766..3984230 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag C5097_RS20975 Protein ID WP_001307405.1
Coordinates 3983431..3983766 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5097_RS20960 3979216..3979986 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
C5097_RS20965 3980002..3981336 - 1335 WP_089588046.1 galactarate/glucarate/glycerate transporter GarP -
C5097_RS20970 3981711..3983282 + 1572 WP_001273753.1 galactarate dehydratase -
C5097_RS20975 3983431..3983766 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
C5097_RS20980 3983766..3984230 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
C5097_RS20985 3984285..3985094 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
C5097_RS20995 3985343..3986623 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
C5097_RS21000 3986646..3987119 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
C5097_RS21005 3987130..3987909 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
C5097_RS21010 3987899..3988777 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
C5097_RS21015 3988795..3989229 + 435 WP_000948842.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3974509..3984230 9721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T97286 WP_000347273.1 NZ_CP027105:3983766-3984230 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T97286 NZ_CP027105:3983766-3984230 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT97286 WP_001307405.1 NZ_CP027105:3983431-3983766 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT97286 NZ_CP027105:3983431-3983766 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References