40091

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 614541..615340 Replicon chromosome
Accession NZ_AP025209
Organism Escherichia coli strain 2017.06.04CC

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LPH28_RS02985 Protein ID WP_000347273.1
Coordinates 614541..615005 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LPH28_RS02990 Protein ID WP_001307405.1
Coordinates 615005..615340 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH28_RS02955 (609542) 609542..609976 - 435 WP_000948814.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LPH28_RS02960 (609994) 609994..610872 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LPH28_RS02965 (610862) 610862..611641 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LPH28_RS02970 (611652) 611652..612125 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LPH28_RS02975 (612148) 612148..613428 - 1281 WP_112044003.1 tagatose-bisphosphate aldolase subunit KbaZ -
LPH28_RS02980 (613677) 613677..614486 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LPH28_RS02985 (614541) 614541..615005 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LPH28_RS02990 (615005) 615005..615340 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LPH28_RS02995 (615489) 615489..617060 - 1572 WP_001273753.1 galactarate dehydratase -
LPH28_RS03000 (617435) 617435..618769 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LPH28_RS03005 (618785) 618785..619555 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 614541..626215 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T40091 WP_000347273.1 NZ_AP025209:c615005-614541 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T40091 NZ_AP025209:c615005-614541 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT40091 WP_001307405.1 NZ_AP025209:c615340-615005 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT40091 NZ_AP025209:c615340-615005 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References