171575

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2329638..2330437 Replicon chromosome
Accession NZ_CP060928
Organism Escherichia coli strain EC13

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H9200_RS11435 Protein ID WP_000347273.1
Coordinates 2329973..2330437 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9200_RS11430 Protein ID WP_001307405.1
Coordinates 2329638..2329973 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9200_RS11415 (2325423) 2325423..2326193 - 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -
H9200_RS11420 (2326209) 2326209..2327543 - 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
H9200_RS11425 (2327918) 2327918..2329489 + 1572 WP_001723936.1 galactarate dehydratase -
H9200_RS11430 (2329638) 2329638..2329973 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9200_RS11435 (2329973) 2329973..2330437 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9200_RS11440 (2330492) 2330492..2331301 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9200_RS11445 (2331550) 2331550..2332830 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9200_RS11450 (2332853) 2332853..2333326 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9200_RS11455 (2333337) 2333337..2334116 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9200_RS11460 (2334106) 2334106..2334984 + 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
H9200_RS11465 (2335002) 2335002..2335436 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2320491..2330437 9946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T171575 WP_000347273.1 NZ_CP060928:2329973-2330437 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T171575 NZ_CP077978:c154256-153732 [Pseudomonas aeruginosa]
ATGAAAATCATCGGAGTGCAGGACCTCCCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACCCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTTGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGGAAGCGCATTCCTTCCACCAGGTCTGCTGGTCAGCTATTCCCTCAGTCTGGACCGAGTGATC
GACTTCACCGGCGGCTATGACGATACCTGGGCCCCGATCTGGCAAGACTTTTTCTGCGACTGGAGAAAGATTCGCTTCAA
CGACGGCGTTGAACCGCCGAGCTGGATTGTCGGGGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTAAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGACGCGCCCGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171575 WP_001307405.1 NZ_CP060928:2329638-2329973 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171575 NZ_CP077978:c154648-154253 [Pseudomonas aeruginosa]
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCTCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACCGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAAGCCTACATTCGTGATTCAGTGAAGGTTCTGCGTGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCAACTACAAGACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCCTGGGAAGCTGGTGCGCAAGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References