37143

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 604978..605777 Replicon chromosome
Accession NZ_AP023286
Organism Escherichia coli strain 2018-18.25PC

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JMH23_RS02900 Protein ID WP_000347273.1
Coordinates 604978..605442 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JMH23_RS02905 Protein ID WP_001307405.1
Coordinates 605442..605777 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMH23_RS02870 599994..600428 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
JMH23_RS02875 600446..601324 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JMH23_RS02880 601314..602078 - 765 WP_032292471.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JMH23_RS02885 602089..602562 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JMH23_RS02890 602585..603865 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JMH23_RS02895 604114..604923 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JMH23_RS02900 604978..605442 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JMH23_RS02905 605442..605777 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JMH23_RS02910 605926..607497 - 1572 WP_001273753.1 galactarate dehydratase -
JMH23_RS02915 607872..609206 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JMH23_RS02920 609222..609992 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 604978..616652 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T37143 WP_000347273.1 NZ_AP023286:c605442-604978 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T37143 NZ_AP023286:c605442-604978 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT37143 WP_001307405.1 NZ_AP023286:c605777-605442 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT37143 NZ_AP023286:c605777-605442 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References