Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 609157..609956 Replicon chromosome
Accession NZ_CP095125
Organism Escherichia coli strain Iso00270

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag MU416_RS02975 Protein ID WP_000347275.1
Coordinates 609157..609621 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MU416_RS02980 Protein ID WP_001307405.1
Coordinates 609621..609956 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU416_RS02945 (604158) 604158..604592 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MU416_RS02950 (604610) 604610..605488 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MU416_RS02955 (605478) 605478..606257 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
MU416_RS02960 (606268) 606268..606741 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
MU416_RS02965 (606764) 606764..608044 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
MU416_RS02970 (608293) 608293..609102 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MU416_RS02975 (609157) 609157..609621 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MU416_RS02980 (609621) 609621..609956 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MU416_RS02985 (610105) 610105..611676 - 1572 WP_001273752.1 galactarate dehydratase -
MU416_RS02990 (612051) 612051..613385 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MU416_RS02995 (613401) 613401..614171 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 609157..620831 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T241423 WP_000347275.1 NZ_CP095125:c609621-609157 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T241423 NZ_LT992464:c1577669-1577562 [Staphylococcus aureus]
GTGATATCTATTGCAAATGCATTACATTTAATGTTAAGTTTCGGTATGTTTATCGTCACTTTCATTGGTGTAGTAGTCGC
AATAATTAATTTAAACAATAAAAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT241423 WP_001307405.1 NZ_CP095125:c609956-609621 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT241423 NZ_LT992464:1577472-1577509 [Staphylococcus aureus]
AACATGTCGCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References