215230

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 629449..630248 Replicon chromosome
Accession NZ_CP082824
Organism Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag K8B79_RS03045 Protein ID WP_000347275.1
Coordinates 629449..629913 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K8B79_RS03050 Protein ID WP_001307405.1
Coordinates 629913..630248 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B79_RS03015 (624450) 624450..624884 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K8B79_RS03020 (624902) 624902..625780 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K8B79_RS03025 (625770) 625770..626549 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
K8B79_RS03030 (626560) 626560..627033 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
K8B79_RS03035 (627056) 627056..628336 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
K8B79_RS03040 (628585) 628585..629394 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K8B79_RS03045 (629449) 629449..629913 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K8B79_RS03050 (629913) 629913..630248 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K8B79_RS03055 (630397) 630397..631968 - 1572 WP_001273752.1 galactarate dehydratase -
K8B79_RS03060 (632343) 632343..633677 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K8B79_RS03065 (633693) 633693..634463 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 629449..641123 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T215230 WP_000347275.1 NZ_CP082824:c629913-629449 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T215230 NZ_CP110615:2827547-2827939 [Rhodococcus sp. 75]
GTGAGCGCGGTGGTGCTCGACACCCACGTCCTGCACTGGTGGTCCGCCGAGCCCGAGCGGCTCAGCGCGCGGGCCGCACG
TGCCGTGGAGGCGGCCGACGAGCTCGTGGTCGCCGGGATCACCTGGTACGAGCTCGCCTGGCTGGCCCGGCACGACCGCA
TCCGCCTCGCCGTCCCGCTCACCGCCTGGCTCGACCGGCTCGCCACCGAGGTCCGCACCGTCGGGATCACCCCGGCTGTC
GCGGTCGCGGCGACCGCGCTGCCGGCGTCGTTCCCCGGGGACCCGGCCGACCGTCTCATCTACGCCACGGCCGTGGAGCA
CGGCTGGGACCTGGTCACCAAGGATCGACGGTTGCACGAGCACGTCCACCCCCGGCCGGTCACGGTCTGGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT215230 WP_001307405.1 NZ_CP082824:c630248-629913 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT215230 NZ_CP110615:2827311-2827550 [Rhodococcus sp. 75]
GTGACCGAGAGGATGAGCGCCAGCACGGCGAAGGCCACGCTGCTGTCCGTGCTCGACCGGGTGGCGGGCGGGGACGAGGT
CGAGATCACCAAGCACGGTCGGGTCGTCGCGAGGCTCGTCCCCGCCAACTCACCGCACGCGCTGCGTGGCCTGCTCTCCG
GCGTCGCCAGCACCGTCGGTACCGATGACGACGTGTTCTCCACCGGTGGCACCTGGGACACGGGGAACGTCCCGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References