257235

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4058640..4059439 Replicon chromosome
Accession NZ_CP104114
Organism Escherichia coli strain USDA-ARS-USMARC-51408

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag N1706_RS19535 Protein ID WP_000347275.1
Coordinates 4058975..4059439 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N1706_RS19530 Protein ID WP_001307405.1
Coordinates 4058640..4058975 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1706_RS19515 (4054425) 4054425..4055195 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
N1706_RS19520 (4055211) 4055211..4056545 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
N1706_RS19525 (4056920) 4056920..4058491 + 1572 WP_001273753.1 galactarate dehydratase -
N1706_RS19530 (4058640) 4058640..4058975 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N1706_RS19535 (4058975) 4058975..4059439 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N1706_RS19540 (4059494) 4059494..4060303 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N1706_RS19545 (4060552) 4060552..4061832 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
N1706_RS19550 (4061855) 4061855..4062328 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
N1706_RS19555 (4062339) 4062339..4063118 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
N1706_RS19560 (4063108) 4063108..4063986 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
N1706_RS19565 (4064004) 4064004..4064438 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4049492..4059439 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T257235 WP_000347275.1 NZ_CP104114:4058975-4059439 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT257235 WP_001307405.1 NZ_CP104114:4058640-4058975 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References